Coding

Part:BBa_J32009:Design

Designed by: Austen Heinz   Group: iGEM06_Duke   (2006-07-16)


4HT Dependent Intein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 522
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1054
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 656
    Illegal XhoI site found at 1180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 522
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 522
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 556


Design Notes

Removal of multiple BB restriction sites.


Source

Allen R. Buskirk, Yi-Ching Ong, Zev J. Gartner, and David R. Liu "Directed evolution of ligand dependence: Small-molecule-activated protein splicing" PNAS 101: 10505-10510

References